New 16 to 48 hour reading window

Specificity: 96.1%

Compatability: 95.9%

Sensitivity: 95.2%

For Sample reading between 16-22 hours: Add Colitag™ to 100mL water sample. Place vessel in 44.5C waterbath for 7 to 10minutes. Incubate for 16 to 22 hours at 35C. Visually check sample for yellow color. No, sample is negitive. Yes, florecence checked using long wave-length ultra-violet lamp. No, sample is negative. Yes. sample is positive for E.coli bacteria

Traditional EPA Protocol 22-48 hours: Add Colitag™ to 100mL water sample. Incubate for 24hours at 35C. Visually check sample for yellow color. No, sample is negative. Yes, flourecence checked using long wave-length ultra-violet lamp. No, sample is negative. Yes Sample is positive for E.coli bacteria.